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used time-resolved fluorescence anisotropy to analyze the 8 kDa protein kinase inhibitor (PKI alpha) of cAMP-dependent protein kinase (cAPK) free in solution and bound to cAPK (Hauer et al., 1999)

Biochem J 87:553559 Hiramoto K, Satoh H, Suzuki T, Moriguchi T, Pi J, Shimosegawa T, Yamamoto M (2014) Myeloid lineage-specific deletion of antioxidant system enhances tumor metastasis

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49,54 In addition, the large size of mRNA and its susceptibility to degradation by nucleases result in low cellular uptake, further limiting its application