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A primary drawback of subcritical water extraction is the requirement for high temperatures, typically between 100C and 374C, which can cause thermal degradation of heat-sensitive polyphenolic compounds, thereby reducing their yield and bioactivity ( Moreover, at elevated temperatures, subcritical water extraction tends to be less selective, extracting a wider range of plant matrix components, which can complicate downstream purification ( Subcritical water extraction equipment requires more rigorous maintenance and corrosion prevention due to the high reactivity and corrosiveness of water at elevated temperature and pressure ( 3.1.6 Pulsed electric field method A nonthermal method that employs high-voltage pulses between two electrodes arranged in a sandwich configuration

A BoxBehnken design (BBD, 3-factor, 3-level) was employed to optimize UAE