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Pellets were incubated in 0.5 mL of ACK buffer for 5 min and centrifuged at 500 g for 10 min at 4 C

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Activated Caspase-3 cleaves gasdermin E (GSDME), releasing its N-terminal pore-forming domain (PFD) from the C-terminal inhibitory structure, thereby forming membrane pores and inducing GSDME-mediated pyroptosis (46)

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