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Drosophila S2R+ cells were obtained from the Drosophila Genomic Research Centre (DGRC) and maintained at 25C

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Standard maintenance therapy typically involves hydroxocobalamin 1mg injections every two to three months for patients without neurological involvement

The abdomen is often favored because it is easy to reach and pinch

Mitochondrial function regulators, including oligomycin, carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP), and a mixture of rotenone and antimycin A (AA/Rot), were sequentially added at the indicated time points