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In brief, hPSCs were plated at a density of 5000 cells/well in a 24-well plate in mTeSR1 medium (Stem Cell Technologies, Vancouver, BC, Canada) + 10 M Y27632 (LC Laboratories, Woodburn, MA) on glass plates (LabTek) coated with 3% GelTrex (Thermo Fisher Scientific, USA) (Day-3)

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Usage rate: 1-3% Product appearance: dark blue liquid Solubility: Can dissolve in water Storage: Store in the refrigerator at 4 C -8 C

16 Participants Participants were recruited using advertisements in social media support groups for individuals with vitamin B12 deficiency/pernicious anaemia (see Acknowledgements)

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