Electrochemical measurements (cyclic voltammetry and chronocoulometry) were performed using a three electrode system with Ag/AgCl as a reference electrode, Pt wire as a counter electrode and a gold disk electrode (r = 1 mm) as a working electrode (CHI Instruments 6273C and CHI420a electrochemical analyzer)
The mechanism story is broad, but most of it is built on cell and animal models
7.2 Relaxin-3 and the behavioral dimension of metabolic disease Relaxin-3, a member of the relaxin peptide family predominantly expressed in the nucleus incertus, signals through the RXFP3 receptor and exerts pronounced effects on feeding behavior, arousal, stress responsiveness, and cognitive processing in animal models (Smith et al., 2014)
Chang M, Wang M, Wang M, Shu M, Ding B, Li C, et al
LeenaarsCHCKouwenaarCStafleuFRBleichARitskes-HoitingaMDe VriesRBMet al