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By contrast, genetic ablation of complex I, which does not trigger AMPK activation, uniformly increases ferroptosis susceptibility by eliminating the compensatory antiferroptotic pathway

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Then, NaCl and PEG 8000 were added into the clarified lysates to reach final concentrations of 200 mM and 10% ( w / v ), respectively, and the mixtures were stirred gently at 4 C overnight to allow protein precipitation