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For 3T3-L1 cells, differentiation was initiated upon confluence (day 0) with DMEM containing 0.5 mM isobutylmethylxanthine (IBMX), 1 M dexamethasone, and 10 g/mL insulin

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Their use for Alcohol Use Disorder (AUD) or drug addiction is considered "off-label." However, groundbreaking clinical research from 2025 and 2026 demonstrates that these medications significantly dampen the brain's reward centers, drastically curbing cravings for alcohol, opioids, and nicotine