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On day 4, cells were resuspended in fresh selection media (RPMI 1640 with 2mM L-glutamine (Gibco), 5% FBS (Gibco), 1x Antibiotic/Antimycotic (Gibco), 5 g/ml Blasticidin (Gibco), and 0.5 g/ml Puromycin (Gibco))

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