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The next day, the samples were washed twice in 100% methanol for 30 min, cooled down to 4 C in 1 h and bleached in chilled fresh 5% H 2 O 2 in methanol (one volume 35% H 2 O 2 to six times volume methanol) overnight at 4 C

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Under IL-1 stimulation, chondrocyte mitochondrial membrane potential decreases substantially, closely correlating with mitochondrial dysfunction (Chen B

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