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The filaments, ranging from 0.04 g to 6.0 g and starting at 0.4 g, were applied to the plantar surface of the hind paw to determine the 50% force withdrawal threshold, using the classic up-down method as previously described 25

cloudiness suggests contamination or precipitation Visible particles : Floating particles, sediment, or crystallisation indicate the medication has degraded Frosted appearance : Suggests the pen has been frozen, rendering it unsafe regardless of expiry date Physical damage to the pen also compromises safety