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Briefly, cells were lysed in PBS containing 1% Triton and 1 mM phenylmethylsulfonyl fluoride (PMSF), along with a complete set of EDTA-free protease inhibitors (Thermo Scientific, Rockford, IL, USA)

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Most of the peptides exhibit a cationic charge combined with an amphipathic character

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Both are valid, but they are not identical