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After progressive heating at a rate of 4 C./min under magnetic stirring, a short interval of transparency should occur close to 70 C., and the inverted phase (water droplets in oil) obtained at 85 C

[DOI] [PubMed] [Google Scholar] 47.Cormia FE, Ernyey A

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There is no single correct amount more water simply spreads the same 10 mg of peptide across a larger volume, which makes small doses easier to measure accurately

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