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At the indicated time points, 1 L of reaction was withdrawn, diluted 6-fold in Replication Stop Dye (3.6% SDS, 18 mM EDTA, 90 mM Tris-HCl pH 8, 90 mg/mL Ficoll, and 3.6 mg/mL Bromophenol Blue), incubated with 20 mg proteinase K at 21C for 16 h, and then resolved by 0.8% agarose gel electrophoresis
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