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[46] This theory has been challenged due to the high level of reactivity of the two free sulfhydryls, low intracellular concentrations of DHLA as well as the rapid methylation of one or both sulfhydryls, rapid side-chain oxidation to shorter metabolites and rapid efflux from the cell

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The study determined the half-maximal inhibitory concentrations (IC50) of metformin and simvastatin using the CCK-8 assay

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