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After 48 h, the cells were washed with PBS (Gibco) and incubated in the assay buffer (HBSS supplemented with Ca 2+ and Mg 2+ (Gibco), 10 mM HEPES (Gibco), 0.1% Pluronic F-68 (Gibco) and 500 M IBMX (Sigma), pH 7.4) with different concentrations of GLP-1 (0 M to 1 M) in the presence (100 nM and 1000 nM) or absence of Fab7F38 at 37 C for 30 min
The order of the food system players was randomized to prevent ordering effects
Although an individual can live without a gallbladder, it does not mean that an individual is not entitled to compensation for the loss of this organ and the pain and suffering associated with the surgery
It may be more effective and cause fewer side effects to use different treatments in rotation rather than just sticking to one
a - g , Supplementary analyses of RNA sequencing data shown in Fig
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